The synthesis of any macromolecule proceeds in three stages: initiation, elongation and termination. DNA ligase joins single-stranded breaks in double-stranded DNA during DNA replication, repair, and recombination while DNA polymerase adds complementary DNA nucleotides to a growing strand in the 5′ to 3′ direction during DNA replication. How does the DNA polymerase and DNA ligase function in DNA replication?įunction. DNA is usually double-stranded, whereas RNA is usually single-stranded. – DNA contains five-carbon sugars, whereas RNA contains six-carbon sugars. – DNA contains nitrogenous bases, whereas RNA contains phosphate groups. How does DNA RNA differ mastering biology? Helicase acts on the lagging strand to unwind dsDNA. Which of the following is true regarding Okazaki fragment? Okazaki fragments are segments of DNA attached to an RNA initiator component. Synthesis of an Okazaki fragment begins with a primer RNA-DNA made by polymerase (Pol) α-primase. Unlike leading strands, lagging strands are synthesized as discrete short DNA fragments, termed ‘Okazaki fragments’ which are later joined to form continuous duplex DNA. What is the role of DNA polymerase during DNA synthesis? DNA polymerase is the enzyme that catalyzes the addition of a nucleotide onto the 3′ end of a growing DNA strand. What is the role of DNA polymerase during DNA synthesis mastering biology quizlet? Okazaki fragments are formed on the lagging strand for the synthesis of DNA in a 5′ to 3′ direction towards the replication fork. Okazaki fragments are formed on lagging strands, initiated by the creation of a new RNA primer by the primosome. They are complementary to the lagging template strand, together forming short double-stranded DNA sections. Okazaki fragments are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication. What are Okazaki fragments and how are they formed quizlet? They are important because they allow for both daughter strands to be synthesized, which are necessary for cell division. Okazaki fragments are small sections of DNA that are formed during discontinuous synthesis of the lagging strand during DNA replication. What are Okazaki fragments and how are they formed? A higher-than-normal rate of DNA synthesis errors.
How does DNA synthesis occur, and what is the direction of synthesis? DNA synthesis occurs in the 5′ to 3′ direction because the phosphate of an incoming dNTP is linked to the 3′ OH group of the growing strand.
How does DNA synthesis occur and what is the direction of synthesis? DNA pol III adds nucleotides to the 3′ end of the lagging strand so that it elongates away from the replication fork. In contrast, the lagging strand is made in segments, each with its own RNA primer. What is the function of DNA polymerase III mastering biology?ĭNA pol III adds nucleotides to the 3′ end of the leading strand so that it elongates toward the replication fork. DNA polymerase catalyzes the synthesis of the template strand of DNA. 24 Which enzyme synthesizes Okazaki fragments in eukaryotic cells? What is the role of DNA polymerase during DNA synthesis mastering biology?ĭNA polymerase is the enzyme that catalyzes the addition of a nucleotide onto the 3′ end of a growing DNA strand.
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